卵母细胞
体内
生物
男科
基因表达
体外成熟
配子
基因
胚胎
卵泡发生
细胞生物学
胚胎发生
胚泡
基因表达谱
人类受精
遗传学
医学
作者
Leony Cristina Caetano,Carolina Gennari Verruma,F.L.V. Pinaffi,Izabelle Jardim,Gilvan Pessoa Furtado,Luciano Andrade Silva,Cristiana Libardi Miranda Furtado,Ana Carolina Japur de Sá Rosa‐e‐Silva
出处
期刊:Zygote
[Cambridge University Press]
日期:2022-10-20
卷期号:31 (1): 31-43
被引量:1
标识
DOI:10.1017/s0967199422000478
摘要
Oocyte gene expression is a well controlled event that promotes gamete competence to undergo maturation, fertilization, and to support early embryo development, directly affecting reproductive outcomes. Considering that in vivo controlled ovarian stimulation or in vitro maturation (IVM) for the acquisition of mature oocytes has distinct implications for gene expression, we sought to evaluate the effects of these procedures on the expression of competence-related genes in single-cell oocytes. Healthy Nelore cows of reproductive age were synchronized to harvest in vivo matured oocytes; ovaries from slaughtered animals were used to obtain cumulus-oocyte complexes that were in vitro matured. Single-cell gene expression was performed using TaqMan Low-Density Arrays and 42 genes were evaluated. In silico analysis of protein interactions and Gene Ontology (GO) analysis was performed. Reduced gene expression was observed for 24 targets in IVM oocytes when compared with those of in vivo matured oocytes (P < 0.05). Differences ranged from 1.5-fold to 4.8-fold higher in in vivo oocytes and the BMP15 (5.28), GDF9 (6.23), NOBOX (7.25), HSPA8 (7.85) and MSX1 (11.00) showed the greatest fold increases. The strongest score of functional interactions was observed between the CDC20 and CKS2, with the differentially expressed gene CDC20 being the main marker behind GO enrichment. IVM negatively affected the expression of important genes related to oocyte competency, and showed higher expression levels in in vivo matured oocytes. In vivo controlled ovarian stimulation may be a better strategy to achieve proper oocyte competence and increase the success of assisted reproductive technologies.
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