Recent advances in the development and analytical applications of oxidase-like nanozymes

Nanozymes are a class of nanomaterials with biological enzyme activity, which have both the merits of nanomaterials and biological enzymes. Although great progress in nanozyme-enabled analytical chemistry has been made, most nanozyme-based sensing platforms are based on peroxidase-like nanozymes. The peroxidase-like nanozymes with multi-enzymatic activities affect detection sensitivity and accuracy, while the use of unstable H2O2 in a peroxidase-like catalytic reaction may result in the reproducibility challenge of sensing signals. Constructing sensing systems by using oxidase-like nanozymes can address these limitations, while insufficient activity and unclear catalytic mechanism limit the analytical applications of oxidase-like nanozymes. In this review, we summarize in detail the development and analytical applications of oxidase-like nanozymes. Possible mechanism of the oxidase-like activity is discussed. Finally, the opportunities and challenges faced by oxidase-like nanozymes are discussed, and it is conducive to developing high activity oxidase-like nanozymes and expanding their analytical applications.