非核糖体肽
聚酮合酶
聚酮
化学
基因组
氨基酸
生物化学
肽
酶
酰基载体蛋白
立体化学
基因
生物合成
作者
Lin Chen,Lin Chen,Xin Wang,Yi Zou,Man-Cheng Tang,Man-Cheng Tang
标识
DOI:10.1021/acs.orglett.4c01039
摘要
Guided by the retrobiosynthesis hypothesis, we characterized a fungal polyketide synthase–nonribosomal peptide synthetase (PKS–NRPS) hybrid megasynthetase pathway to generate 2-trans-4-trans-2-methylsorbyl-d-leucine (1), a polyketide amino acid conjugate that inhibits Arabidopsis root growth. The biosynthesis of 1 includes a PKS–NRPS enzyme to assemble an N-acyl amino alcohol intermediate, which is further oxidized to an N-acyl amino acid (NAAA), demonstrating a new biosynthetic logic for synthesizing NAAAs and expanding the chemical space of products encoded by fungal PKS–NRPS clusters.
科研通智能强力驱动
Strongly Powered by AbleSci AI