光学
显微镜
微分干涉显微术
材料科学
摄影术
相衬显微术
方解石
相(物质)
光学显微镜
单发
显微镜
梁(结构)
衍射
扫描电子显微镜
物理
化学
矿物学
量子力学
作者
Anuj Saxena,Azeem Ahmad,Vishesh Dubey,Hong Mao,Anand Kumar,Anowarul Habib,Satish Kumar Dubey,Balpreet Singh Ahluwalia,Anuj Saxena
出处
期刊:Applied Optics
[Optica Publishing Group]
日期:2024-10-14
卷期号:63 (32): 8350-8350
被引量:1
摘要
This paper presents the development of a single-shot, partially spatially coherent quantitative differential phase contrast microscopy (Q-DPCM) system. The optical scheme comprises a polarizer, lenses, calcite beam displacer, and analyzer, which can be seamlessly integrated to an existing bright-field microscopy system, transforming it into a Q-DPCM system. It utilizes a partially spatially coherent light source, enabling single-shot quantitative differential phase recovery of the specimens with high spatial phase sensitivity. It generates highly sensitive quantitative differential phase images of the specimens along one direction, like a gradient light interference microscopy (GLIM) system, using only a single interferogram. First, we validated the differential phase measurement capability of the system through experiments on polystyrene spheres (diameter 5.2 µm) and HeLa cells. Next, the system is utilized to generate quantitative phase maps of human red blood cells using two orthogonal differential interferograms recorded at two orientations of the calcite beam displacer. Further, the Q-DPCM system is implemented for 1-h time-lapse live cell monitoring, revealing the dynamics of intracellular granules such as nucleolus and lipids in U2OS cells.
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