Release of Peroxidase from Eosinophil Granules in vitro

THE eosinophil is a phagocytic cell1, and following the ingestion by eosinophils of sensitized red cells many of the eosinophil granules disappear2. Histochemical studies recently have shown that eosinophil granules contain significant amounts of the enzyme peroxidase3. The conditions necessary for lysis of the granules and release of peroxidase were investigated in the present work, using preparations of eosinophils isolated from the peritoneal cavity of the rat.