Multicomponent Analysis of Highly Overlapped Capillary Electrophoretic Peaks Using Multiwave-Length Charge-Coupled Devices Detection

A chemometric method, Kalman filtering, was applied to deal with three-dimensional electropherograms, obtained by capillary electrophoresis connected to a charge-coupled device multiwavelength fluorescence detector. The capillary electrophoretic peaks of a mixture, which were not effectively separated, were resolved into electrophoretic peaks of individual components to obtain qualitative and quantitative information. The approach has been applied to analyze a mixture of rhodamine fluorescent dyes with excellent results.