瘤芽
波形蛋白
上皮-间质转换
医学
免疫组织化学
细胞角蛋白
病理
胰腺癌
腺癌
肿瘤科
转移
淋巴结
癌症
癌症研究
内科学
淋巴结转移
作者
Ilona Kohler,Peter Bronsert,Sylvia Timme,Martin Werner,Thomas Brabletz,Ulrich T. Hopt,Oliver Schilling,Dirk Bausch,Tobias Keck,Ulrich Wellner
摘要
Abstract Background and Aim Pancreatic ductal adenocarcinoma ( PDAC ) is characterized by aggressive biology and poor prognosis even after resection. Long‐term survival is very rare and cannot be reliably predicted. Experimental data suggest an important role of epithelial‐mesenchymal transition ( EMT ) in invasion and metastasis of PDAC . Tumor budding is regarded as the morphological correlate of local invasion and cancer cell dissemination. The aim of this study was to evaluate the biological and prognostic implications of EMT and tumor budding in PDAC of the pancreatic head. Methods Patients were identified from a prospectively maintained database, and baseline, operative, histopathological, and follow‐up data were extracted. Serial tissue slices stained for Pan‐Cytokeratin served for analysis of tumor budding, and E ‐Cadherin, Beta‐Catenin, and Vimentin staining for analysis of EMT . Baseline, operative, standard pathology, and immunohistochemical parameters were evaluated for prediction of long‐term survival (≥ 30 months) in uni‐ and multivariate analysis. Results Intra‐ and intertumoral patterns of EMT marker expression and tumor budding provide evidence of partial EMT induction at the tumor–host interface. Lymph node ratio and E ‐Cadherin expression in tumor buds were independent predictors of long‐term survival in multivariate analysis. Conclusions Detailed immunohistochemical assessment confirms a relationship between EMT and tumor budding at the tumor–host interface. A small group of patients with favorable prognosis can be identified by combined assessment of lymph node ratio and EMT in tumor buds.
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