免疫系统
化学
肿瘤坏死因子α
MAPK/ERK通路
一氧化氮
药理学
生物化学
免疫学
生物
激酶
内分泌学
作者
Mi Hyeon Jang,Tae‐Gyu Lim,Sungeun Ahn,Hee‐Do Hong,Young Kyoung Rhee,Kyung‐Tack Kim,Eunjung Lee,Jeong Hoon Lee,Yun Ji Lee,Chan Sik Jung,Dong Hoon Lee,Chang‐Won Cho
出处
期刊:Nutrients
[MDPI AG]
日期:2016-09-27
卷期号:8 (10): 600-600
被引量:24
摘要
The objective of this study was to investigate the immune-enhancing activity of a high molecular weight fraction (HMF) of Cynanchum wilfordii in RAW 264.7 macrophages and the cyclophosphamide (CYC)-induced mouse model of immunosuppression. To identify the bioactive substances of HMF, a crude polysaccharide (HMFO) was obtained and treated with sodium periodate (an oxidation agent) or digested with protease. In macrophages, HMF treatment enhanced the production of nitric oxide (NO) and cytokines (tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β)), as well as phagocytic ability. In CYC-immunosuppressed mice, HMF improved relative spleen and thymus weights, natural killer (NK) cell activity, and splenic lymphocyte proliferation. These increases in NO and cytokines were mediated by up-regulation of nuclear factor kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways. Periodate treatment, but not protease treatment, decreased the immune-enhancing activity of HMFO, suggesting that polysaccharides are the active ingredients in C. wilfordii extract.
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