Evaluation of 18F-SFB-Annexin B1 in detecting apoptosis

：Objective To evaluate18F-N- succinimidyl -4-fluorobenzoate (SFB)-Annexin B1 in detectingin vitro andin vivoapoptosis. Methods Anti-Fas antibody was used to induce apoptosis in Jurkat cells.Apoptosis in Jurkat cells was confirmed by flow cytometer (FCM). Unilateral renalischemia/reperfusion injury was induced by transient (45 min) ligation of the renal arteryin the rabbit. The rabbit was then administrated with 18F-SFB-Annexin B1 intravenously 24h later and then imaged by PET/CT at 10,30,60,90,120 and 240 min postinjection. Apoptosisin kidney was confirmed by terminal deoxynucleotidyl transferase mediated dUTP biotin nickend labeling (TUNEL) assay and HE staining. Results The apoptosis rate induced by anti-Fasantibody was 25.98%(120 min) while that in the control group was only 1.81%. The uptake of18F-SFB-Annexin B1in apoptosis group was greater than that in the control group. PET/CTimages at 240 min showed higher uptake in the ligated kidney than the non-ligated kidney.TUNEL assay and HE staining confirmed great amount apoptotic cells in the ligated kidney.Conclusion 18 F-SFB-Annexin B1may be potentially useful in detecting apoptosis both invitro and in vivo.