Detection of serum antibodies from patients with Schistosoma japonicum by magnetic affinity immunoassay.

To observe the effect of magnetic affinity immunoassay(MPAIA) on detecting the serum antibodies of patients with Schistosoma japonicum. this method of assay was used to detect specific serum antibodies of 384 patients with Schistosoma japonicum. It was found that the false positive rate of 384 sera of healthy persons from non-endemic region was 0. And the positive rate of 61 sera from acute schistosomiasis patients,788 sera from chronic schistosomiasis patients and 32 sera from advanced schistosomiasis patients were 100%,94.2% and 90.6%,respectively. The cross reaction rate of sera from patients infected with other parasites(14 patients with paragonimiasis,13 patients with cysticercosis and 9 patients with trichinosis) was 0.The result shows that MPAIA is a sensitive and specific assay to detect serum antibodies from patients with schistosomiasis and MPAIA can be applied to detect the serum antibodies from patients infected with Schistosoma japonicum.