间充质干细胞
细胞生物学
细胞凋亡
细胞生长
体外
端粒酶
化学
干细胞
细胞
增殖标记
细胞分化
细胞培养
生物
生物化学
遗传学
基因
作者
Yuming Li,Tatjana Schilling,Peggy Benisch,Sabine Zeck,Jutta Meißner-Weigl,Doris Schneider,Catarina Limbert,Jochen Seufert,Moustapha Kassem,Norbert Schütze,Franz Jakob,Regina Ebert
标识
DOI:10.1016/j.bbrc.2007.08.161
摘要
High glucose (HG) concentrations impair cellular functions and induce apoptosis. Exposition of mesenchymal stem cells (MSC) to HG was reported to reduce colony forming activity and induce premature senescence. We characterized the effects of HG on human MSC in vitro using telomerase-immortalized MSC (hMSC-TERT) and primary MSC (hMSC). HG (25mM) enhanced hMSC-TERT proliferation in long-term studies in contrast to hMSC where proliferation was unchanged. Thioredoxin-interacting protein, which is involved in apoptosis regulation, was stimulated by glucose in hMSC-TERT. However, apoptosis was not influenced by HG in both cell types. MSC treatment with HG favored osteogenic differentiation. MSC are resistant to HG toxicity, depending on the stemness of MSC. Proliferation and osteogenic differentiation are stimulated by HG. Effects of HG on the transient amplifying compartment of MSC may differ from those in mature cells. Further research is needed to unravel the molecular mechanisms of HG resistance of MSC.
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