明胶
生物反应器
聚二甲基硅氧烷
表面改性
材料科学
嫁接
粘附
蛋白质吸附
生物污染
细胞粘附
化学工程
微通道
牛血清白蛋白
吸附
纳米技术
色谱法
化学
聚合物
复合材料
生物化学
有机化学
膜
工程类
作者
Fatemeh Sarvi,Zhilian Yue,Kerry Hourigan,Mark C. Thompson,Peggy Chan
摘要
This study presents a novel and inexpensive method to prepare a disposable micro-bioreactor for stem cell expansion. The micro-bioreactor was fabricated in the form of a fixed bed bioreactor with a microchannel reactor bed. The micro-bioreactor was constructed from polydimethylsiloxane (PDMS), and the microchannel was functionalized to enable cell adhesion and resistance to bovine serum albumin protein adsorption. The PDMS reactor bed surface was activated by oxygen plasma, then aminized with trimethoxysilylpropyl(polyethyleneimine), followed by grafting with carboxylmethyl cellulose (CMC) and gelatin in sequence. The functionalized PDMS surface demonstrated improved hydrophilicity and antifouling properties. The grafting of gelatin promoted cell adhesion. The functionalized surface was found to be biocompatible with MDA-MB-231 and Oct4b2 cells and was demonstrated to facilitate cell proliferation. The expanded Oct4b2 cells retained their proliferation potential, undifferentiated phenotype and pluripotency.
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