In Situ Fluorogenic Reaction Generated via Ascorbic Acid for the Construction of Universal Sensing Platform

A highly fluorescent emission reaction between terephthalic acid (PTA) and ascorbic acid (AA) via simple control of the reaction temperature was first revealed with the detailed formation mechanism and various characterizations including electron paramagnetic resonance and mass spectrometry. Based on the AA-responsive emission, the alkaline phosphatase (ALP) triggered the transformation of l-ascorbic acid 2-phosphate trisodium salt to AA was integrated with the present system for developing a sensitive, selective, and universal platform. The monitoring of the activity of ALP and the fabrication of ALP-based enzyme-linked immunoassay (ELISA) with carcinoembryonic antigen (CEA) as the model target was performed. The fluorescence intensity correlated well to the CEA concentration in the ranges of 0.25-30 ng/mL, with a detection limit of 0.08 ng/mL. Such a facile protocol based on the fluorescent reaction between PTA and AA without the assistance of catalysis of nanomaterials avoided the laborious synthesis procedure and provided a direct strategy for the early clinical diagnosis coupled with ALP-related catalysis.