Cyclophilin B promotes cell proliferation, migration, invasion and angiogenesis via regulating the STAT3 pathway in non-small cell lung cancer

血管生成 基因沉默 细胞生长 肺癌 癌症研究 免疫印迹 生物 A549电池 细胞迁移 癌症 细胞 医学 病理 遗传学 基因
作者
Mao-rong Teng,Jianan Huang,Zhengtai Zhu,Hua Li,Jiang-feng Shen,Quan Chen
出处
期刊:Pathology Research and Practice [Elsevier BV]
卷期号:215 (6): 152417-152417 被引量:16
标识
DOI:10.1016/j.prp.2019.04.009
摘要

Lung cancer is the most common type of cancer and has become the leading cause of cancer-associated mortality worldwide. It has been reported that expression of Cyclophilin B was greatly elevated in the pancreatic cancer patient sera as compared with the healthy volunteer sera. This study aimed to investigate the role and regulatory mechanism of CypB in NSCLC progression. The expression levels of CypB was detected in NSCLC samples and cell lines by ELISA, western blot and immunohistochemistry assay. In addition, CCK8, colony formation, scratch and transwell assays were used to evaluate the proliferation, migration and invasion of A549 cells with CypB silencing. The expression of angiogenesis related proteins and pathway-related factors were detected by western blot. In NSCLC samples, CypB expression was upregulated. The expression of CypB was significantly reduced in the siRNA-cyclophilin B group. In addition, CypB silencing inhibited cell proliferation, migration and invasion. The expression of angiogenesis related proteins and pathway-related factors have also changed significantly. These findings suggested that CypB silencing may suppress the proliferation, invasion, migration and angiogenesis of A549 cells via inhibiting STAT3 pathway.

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