Endoglycosidase and Glycoamidase Release of N‐Linked Oligosaccharides

Abstract Carbohydrate chain modifications are often used to monitor glycoprotein movement through the secretory pathway. This is because stepwise sugar‐chain processing is unidirectional and generally corresponds to the forward or anterograde movement of proteins. This unit presents a group of techniques for analysis of the general structure of carbohydrate chains on a protein and, therefore, oligosaccharide processing mileposts. It has been revised from a previous version to include endoglycosidase H, D, F 2 , and F 3 digestions, as well as peptide:N‐glycosidase F (PNGase F), neuraminidase, endo‐β‐galactosidase, and endo‐α‐N‐acetylgalactosaminidase digestions.