Nano-enabled sensing approaches for pathogenic bacterial detection

Infectious diseases caused by pathogenic bacteria, especially antibiotic-resistant bacteria, are one of the biggest threats to global health. To date, bacterial contamination is detected using conventional culturing techniques, which are highly dependent on expert users, limited by the processing time and on-site availability. Hence, real-time and continuous monitoring of pathogen levels is required to obtain valuable information that could assist health agencies in guiding prevention and containment of pathogen-related outbreaks. Nanotechnology-based smart sensors are opening new avenues for early and rapid detection of such pathogens at the patient's point-of-care. Nanomaterials can play an essential role in bacterial sensing owing to their unique optical, magnetic, and electrical properties. Carbon nanoparticles, metallic nanoparticles, metal oxide nanoparticles, and various types of nanocomposites are examples of smart nanomaterials that have drawn intense attention in the field of microbial detection. These approaches, together with the advent of modern technologies and coupled with machine learning and wireless communication, represent the future trend in the diagnosis of infectious diseases. This review provides an overview of the recent advancements in the successful harnessing of different nanoparticles for bacterial detection. In the beginning, we have introduced the fundamental concepts and mechanisms behind the design and strategies of the nanoparticles-based diagnostic platform. Representative research efforts are highlighted for in vitro and in vivo detection of bacteria. A comprehensive discussion is then presented to cover the most commonly adopted techniques for bacterial identification, including some seminal studies to detect bacteria at the single-cell level. Finally, we discuss the current challenges and a prospective outlook on the field, together with the recommended solutions.