A Deep Learning Model for RNA-Protein Binding Preference Prediction based on Hierarchical LSTM and Attention Network

Attention mechanism has the ability to find important information in the sequence. The regions of the RNA sequence that can bind to proteins are more important than those that cannot bind to proteins. Neither conventional methods nor deep learning-based methods, they are not good at learning this information. In this study, LSTM is used to extract the correlation features between different sites in RNA sequence. We also use attention mechanism to evaluate the importance of different sites in RNA sequence. We get the optimal combination of k-mer length, k-mer stride window, k-mer sentence length, k-mer sentence stride window, and optimization function through hyper-parm experiments. The results show that the performance of our method is better than other methods. We tested the effects of changes in k-mer vector length on model performance. We show model performance changes under various k-mer related parameter settings. Furthermore, we investigate the effect of attention mechanism and RNA structure data on model performance.