Activatable Off‐on Near‐Infrared QCy7‐based Fluorogenic Probes for Bioimaging

Abstract The activatable off‐on near‐infrared QCy7‐based fluorogenic probes have emerged as powerful modalities for detecting and monitoring biological analytes and understanding their biological processes in cells and organisms. The use of biomarker‐activated QCy7‐based probes enables simple synthesis, minimum photo‐damage to biological samples, and minimum background interference from biological systems. In this minireview, we aim to provide a rigorous but concise overview of activatable QCy7‐based fluorogenic probes by reporting the significant progress made in recent years. The design strategies and the main applications of accurate detection and imaging of disease‐related biomarkers (including ROS/RSS, enzymes, metal ions, and other related species) were reasonably analyzed and discussed. The potential challenges and prospects of activatable QCy7‐based fluorogenic probes are also emphasized to further advance the development of new methods for biomarker detection and bioimaging.