视网膜色素上皮
光毒性
细胞生物学
p38丝裂原活化蛋白激酶
氧化应激
活性氧
MAPK/ERK通路
化学
细胞损伤
激酶
材料科学
生物物理学
生物
视网膜
生物化学
体外
作者
Jiayin Song,Daoyong Li,Zhongshu Shan,Olga Kurskaya,Kirill Sharshov,Gao Tingting,Hongtao Bi
标识
DOI:10.1016/j.jphotobiol.2020.112058
摘要
WLEDs have lately been the preferred lighting device based on properties such as energy saving, high efficiency, longevity, and environmental protection. However, studies on the safety of white light-emitting diode (WLED) are limited. In our previous study, we found that WLED light (4000 K ± 500 K color temperature, 250 lx, and 20 min exposure) is photocytotoxic to three mammalian cell lines by causing cell lipid peroxidation. To further investigate the potential photocytotoxicity of WLEDs on the human body, we used two human eye cell lines SRA01/04 and D407 as target cells for evaluating its potential phototoxicity on the human eye in the present study based on cell viability, apoptosis, and intracellular oxidative stress assays, as well as the activation levels of reactive oxygen species (ROS)-related apoptosis pathways, including extracellular signal-regulated kinase (ERK), c-Jun NH2-terminal kinase (JNK) and p38 kinase (p38), using mitogen-activated protein kinase (MAPK) signaling pathway assays. The results showed that WLED light has photocytotoxicities on SRA01/04 and D407 cells, which were both in a time-, irradiance-, and color temperature-dependent manner and strongest at the conditions of 2 h irradiation time, 60 W/m 2 irradiance, and 4000 K color temperature. Moreover, the photocytotoxicity of red light-emitting diode (LED) light was the strongest in the three tested monochromatic light compositions of WLED. Mechanism studies show that the potential phototoxicity of WLED on human lens epithelium and retinal pigment epithelium may be caused by its induced oxidative stress damage via the JNK and p38 MAPKs pathways. • WLED was identified to induce photocytotoxicity on human eye cells. • The photocytotoxicity of WLED light is in a time-, irradiance-, and color temperature-dependent manner. • The photocytotoxicity of red LED light was the strongest in the three tested monochromatic light compositions of WLED. • The phototoxicity of WLED may be caused by its induced oxidative stress damage via the JNK and p38 MAPKs pathways.
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