Highly sensitive and simple colorimetric assay of hydrogen peroxide and glucose in human serum via the smart synergistic catalytic mechanism

Due to the own defects of natural enzymes, artificial simulated enzymes are always concerned. Here, the fabricated graphene oxide (GO)/AuNPs nanocomposite exhibits strong synergistic catalysis of peroxidase-mimicking enzymes in combination with the novel property of GO catalytic interface and AuNPs-mediated electron transfer. It can efficiently catalyze the oxidation of enzyme substrate TMB by hydrogen peroxide to form blue TMB oxide. Based on this, the rapid and highly sensitive colorimetric detection of hydrogen peroxide was achieved. Because of the wonderfully synergistic coupling catalysis from GO/AuNPs nanocomposites, the developed artificial enzyme has ultra-strong catalytic activity. For the detection of hydrogen peroxide, the detection limit of this colorimetric analysis is as low as 4.2 × 10−8 M, which is about 1–2 orders of magnitude lower than that of the assays using other single nanoparticles as nanozymes. And it shows high sensitivity. The catalytic oxidation of the prepared nanocomposites to TMB can be completed in minutes, and the response is extremely fast. Combined with the reaction of glucose and glucose oxidase, the colorimetric analysis also realizes the rapid and highly sensitive detection of glucose in human serum. The research results infer that the smart synergy is an effective way to improve the catalytic activity of mimic enzyme. Together with its simplicity in preparation, the GO/AuNPs nanocomposite has excellent development potential in biomedical detection and biosensor design.