MPTP公司
神经退行性变
神经化学
帕金森病
星形胶质增生
多巴胺能
神经科学
医学
多巴胺
疾病
内科学
心理学
中枢神经系统
作者
Ana B. Muñoz-Manchado,Javier Villadiego,Sonia Romo-Madero,Nela Suárez-Luna,Alfonso Bermejo-Navas,José A. Rodríguez‐Gómez,Pablo Garrido‐Gil,José Luis Labandeira-Garcı́a,Miriam Echevarrı́a,José López‐Barneo,Juan José Toledo‐Aral
摘要
Despite the different animal models of Parkinson's disease developed during the last years, they still present limitations modelling the slow and progressive process of neurodegeneration. Here, we undertook a histological, neurochemical and behavioural analysis of a new chronic parkinsonian mouse model generated by the subcutaneous administration of low doses of MPTP (20 mg/kg, 3 times per week) for 3 months, using both young adult and aged mice. The MPTP-induced nigrostriatal neurodegeneration was progressive and was accompanied by a decrease in striatal dopamine levels and motor impairment. We also demonstrated the characteristic neuroinflammatory changes (microglial activation and astrogliosis) associated with the neurodegenerative process. Aged animals showed both a faster time course of neurodegeneration and an altered neuroinflammatory response. The long-term systemic application of low MPTP doses did not induce any increase in mortality in either young adult or aged mice and better resembles the slow evolution of the neurodegenerative process. This treatment could be useful to model different stages of Parkinson's disease, providing a better understanding of the pathophysiology of the disease and facilitating the testing of both protective and restorative treatments. Here, we show a new chronic and progressive parkinsonian mouse model, in young and aged mice. This model produces a stable degeneration of the dopaminergic nigrostriatal pathway, continuous neuroinflammatory reaction and motor deficits. Aged animals showed a faster neurodegeneration and an altered neuroinflammatory response. This treatment could be useful to model different stages of PD and to test both protective and restorative therapeutic approaches.
科研通智能强力驱动
Strongly Powered by AbleSci AI