Crosslinked Polyacrylic Acid as a Carrier for the Immobilization of Bovine Serum Albumin

Polyacrylic acid beads crosslinked with divinyl benzene have been synthesized by suspension polymerization. The continuous phase was 34% aqueous solution of calcium chloride. The discontinuous organic phase was composed of monomer and crosslinking agent and porgenerating solvent, petroleum ether(b.p.90～120 ℃). The initiator, benzoyl peroxide, were added to the monomer prior to suspension . The rate of agitation was controlled at 800 r/min. Polymerization was allowed to proceed for 24 h at 70 ℃. It is an effective carrier for the immobilization of proteins. At optimum molar ratio of acrylic acid to divinyl benzene, high binding amount of the bovine serum albumin on this carrier was obtained.