Mycelial Biomass Exchange in Different Growth Stage Cultures of Flammulina velutipes

Mycelial DNA was used as the parameter to determine fungal biomass in the cultivation substrate during the growth of Flammulina velutipes,strain FV.Substrate DNA levels,their effect on mycelial DNA extraction,and mycelial biomass in bottle cultures,were determined using a standard curve relating mycelial biomass with DNA content.Determination of mycelial biomass in samples collected from the upper,middle and lower layers of bottle cultures at different growth stages indicated a positive correlation between biomass and the‘age'of the mycelium(i.e.growth period from inoculation and sample testing),and that biomass levels continually increased for a certain period after the substrate had been fully colonized.Mycelial biomass in the cultivation substrate decreased during fruit body growth,and occurred earlier and more rapidly in the upper layer compared with the middle and lower layers.