Determination of ginsenoside Rg1,ginsenoside Re_1,ginsenoside Rb1 and ginsenoside Rd_1 in Wushen Xingnao Dropping Pill by HPLC

AIM To establish an HPLC method for determining the contents of ginsenoside Rg1,ginsenoside Re,ginsenoside Rb1 and ginsenoside Rd in Wushen Xingnao Dropping Pill(Ginseng Radix et Rhizoma,Polygoni multiflori Radix,Gingko leaves extract).METHODS Chromatography was performed on a Diamonsil-C18(150 mm×4.6 mm,5.0 μm) at 30 ℃.The mobile phase was composed of acetonitrile and water in gradient mode with a flow rate of 1.0 mL/min.The detection wavelength was set at 203 nm.RESULTS The calibration curves of ginsenoside Rg1,ginsenoside Re,ginsenoside Rb1 and ginsenoside Rd were linear in the ranges of 11.68-58.4 μg/mL,15.30-153.0 μg/mL,13.25-132.5 μg/mL,7.65-76.5 μg/mL,respectively.The average recoveries of ginsenoside Rg1,ginsenoside Re,ginsenoside Rb1,ginsenoside Rd were 97.5%,100.3%,97.6%,98.6%,respectively.And RSDs were 1.56%(n=6),2.13%(n=6),1.98%(n=6),1.53%(n=6),respectively.CONCLUSION The method can be used for quantitative determination of the preparation.