A field plot experiment was conducted to study effects of formula fertilization and local conventional fertilization on yield and fertilizer use efficiency of medium rice variety Yangliangyou No.6 in the river sand mud field in Cili County. The results showed that in comparison with non-fertilization, the yield of Yangliangyou No.6 with formula fertilization and conventional fertilization increased by 86.04% and 82.49%, respectively; the fertilizer use efficiency of N, P and K with formula fertilization was 34.85%, 18.77% and 39.59%, respectively, which increased by 4.71, 1.12 and 0.87 percentage points. Therefore, in comparison with conventional fertilization, formula fertilization is benefit for increasing absorption of N, P and K fertilizer by grain and stem of Yangliangyou No.6.