Masson Trichrome Stain: Postfixation Substitutes

AbstractAbstractFormaldehyde-fixed paraffin-embedded tissues must be post fixed before staining with the Masson's trichrome method. Although Bouin's solution is specified as the chemical of choice, we report on the results of using different post fixatives in the procedure. The use of Gram's iodine or citrate buffer as a postfixative in the Masson's trichrome procedure produces slides that exhibit superior staining qualities than when Bouin's solution or a Bouin's substitute is used. (The J Histotechnol 26:131, 2003)Submitted February 7, 2003; Accepted April 9, 2003Keywords: Gram's iodinecitrate bufferBouin's