[Genetic identification and sequence analysis of three individuals of rare ABO variant Bw subgroup].

To identify and analysis three ABO variant Bw subtypes.Serological assays were carried out to identify the ABO blood group of the proband. ABO gene was identified by Sanger sequencing.The genotype of three individuals are ABO*Bw.11/0.01.02, ABO*Bw.12/0.01.01, ABO*Bw.34/A1.02, receptively. Sequencing results showed that there were c.695T>C, c.278C>T, c.889G>A, resulting in variants in Leu232Pro, Pro93Leu and Glu297Lys, receptively.Bw11, Bw12 and Bw34 subgroups were identified, and gene testing can be used as a supplement to determine the ABO blood group subtypes.