CRISPR Screens

Genetic screening in eukaryotes is being transformed by CRISPR/Cas9 (CRISPR) genome editing technology. This far reaching application of CRISPR technology harnesses its robust, programmable, RNA-guided, DNA genome editing activity for the creation of libraries of mutant eukaryotic cells. Screens identify individuals (or cells) with phenotypes of interest in heterogeneous populations. The first genome scale CRISPR screens consisted of loss of function screens used to uncover genes required for cellular growth and susceptibility to drugs. Several approaches for phenotypic measurement and enrichment have been employed in CRISPR screens. CRISPR screens have been instrumental in the investigation of host pathogen interactions. Numerous bacterial pathogens have complex intracellular life cycles within host cells. CRISPR-based screens still hold untapped potential to further transform and disrupt myriad fields.