蛹虫草
多糖
TLR4型
免疫系统
机制(生物学)
冬虫夏草
对接(动物)
生物
免疫
化学
计算生物学
生物化学
免疫学
医学
信号转导
哲学
食品科学
护理部
认识论
作者
Hongyan Pei,Zhongmei He,Weijia Chen,Yan Zhao,Jianming Li,Ruibing Wang,Ying Zong,Rui Du
摘要
The role of polysaccharide components in the immune system, especially immunomodulatory effects, has received increasing attention. In this context, in this study, network pharmacology was adopted to explore the hypothesis of a multitarget mechanism for immune modulation by Chrysalis polysaccharides. A total of 174 common targets were screened by network pharmacology, with the main ones being TNF, MAPK3, CASP3, VEGFA, and STAT3, mostly enriched in the Toll pathway. The molecular docking results showed that the polysaccharide fraction of Chrysalis binds well to TNF proteins. Besides, in vitro cellular assays were performed to verify the ability of Chrysalis polysaccharides to regulate macrophage polarization and to screen for macrophage surface receptors. Furthermore, in vivo experiments were conducted to prove the activation of TLR4 and TNF-α protein expression in mice by Chrysalis polysaccharide.
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