Genistein Reduced Eosinophilic Inflammation and Remodeling by Downregulating PAI‐1 and TSLP

The data that support the findings of this study are available from the corresponding author upon reasonable request. Figure S1: Schematic representation of experimental design. BALB/c mice were divided into four groups: ovalbumin (OVA) + control mixture/vehicle (Veh), OVA + BIO 300, PBS + Veh, and PBS + BIO 300. PBS groups were sensitized and challenged with PBS. OVA groups were sensitized with 20 μg of OVA by intraperitoneal (i.p) injection on day 0 and day 7. All four groups were pretreated with either vehicle or BIO 300 orally every day from day 14 to day 28. PBS or OVA was administered intranasally from day 14 to day 28. Mice were sacrificed on day 29. Figure S2: Inflammatory cell infiltration in the peribronchiole of OVA mice treated with BIO300. Representative images of H&E staining. Scale bar: 50 μm. Upper panels: 40×, lower panels: zoomed images. The arrows indicate inflammatory cells, including eosinophils, neutrophils, and lymphocytes. Figure S3: Histopathological evaluation of TSLP in the mouse lung with BIO 300 treatment. (A) The IHC examination of TSLP-positive epithelial cells. TSLP-positive cells in OVA+Veh were increased but decreased with BIO 300. (B and C) TSLP staining intensity or cell number was measured in the lung stroma. (B) Intensity of stromal TSLP was increased in OVA+Veh but not significantly reduced in OVA+BIO 300. (C) The cell counts of stromal TSLP showed a trend of increase in OVA+Veh and a trend of decrease in OVA+BIO 300. Data are means ± SE (n = 6–10 mice/group). * p < 0.05 and *** p < 0.001. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.