坏死性下垂
庆大霉素
肾毒性
医学
巨噬细胞
肾
表型
低强度脉冲超声
药理学
免疫学
癌症研究
细胞凋亡
抗生素
化学
内科学
超声波
体外
程序性细胞死亡
治疗性超声
生物化学
基因
放射科
作者
Sarah Ali Abdelhameed Gouda,Basma Emad Aboulhoda,Omaima Mohammed Abdelwahed,Hend Abdallah,Laila Rashed,Rania Elsayed Hussein,Nivin Sharawy
出处
期刊:Life Sciences
[Elsevier]
日期:2023-02-01
卷期号:314: 121338-121338
被引量:8
标识
DOI:10.1016/j.lfs.2022.121338
摘要
Many attempts to control acute kidney injury (AKI) have failed due to a lack of understanding of its pathophysiological key components. Macrophages are a crucial determinant of AKI, which can be categorized functionally as M1 pro-inflammatory and M2 anti-inflammatory macrophages. Low-intensity pulsed ultrasound (LIPUS) is currently being investigated as an immune modulator. The present study aimed to explore the potential effects of LIPUS on the polarization of renal macrophages, as well as the possible interplay between macrophage polarization and necroptosis in gentamicin-induced acute kidney injury.All rats were randomly allocated into one of four groups: control, LIPUS-treated control, gentamicin acute kidney (GM-AKI), and LIPUS-treated GM-AKI. Renal functions, macrophage polarization, necroptosis, and heat shock protein-70 (HSP70) were analyzed using real-time reverse-transcriptase-polymerase chain reaction (rT-PCR), Western Blot, Enzyme-linked immunosorbent assay (ELISA) as well as immunohistological analysis.we found that LIPUS markedly inhibited the expressions of M1 macrophage-related genes and promoted significantly the expression of M2 macrophages related genes. This was accompanied by an inhibition of necroptosis and a marked reduction of HSP-70, resulting in a reversal of gentamicin-induced renal alteration.Functional switching of macrophage responses from M1 into M2 seems to be a potential approach to ameliorate necroptosis as well as HSP-70 by low pulsed ultrasound waves in GM-AKI.
科研通智能强力驱动
Strongly Powered by AbleSci AI