赫拉
细胞
小RNA
细胞内
计算机科学
精密医学
纳米技术
个性化
个性化医疗
接口(物质)
材料科学
化学
癌症研究
计算生物学
生物信息学
医学
生物
生物化学
气泡
病理
最大气泡压力法
万维网
并行计算
基因
作者
Yi Zheng,Si‐Yuan Yu,Zheng Li,Yi‐Tong Xu,Weiwei Zhao,Dechen Jiang,Hong‐Yuan Chen,Jing‐Juan Xu
出处
期刊:Small
[Wiley]
日期:2023-11-16
标识
DOI:10.1002/smll.202307067
摘要
Abstract This work proposes the concept of single‐cell microRNA (miR) therapy and proof‐of‐concept by engineering a nanopipette for high‐precision miR‐21‐targeted therapy in a single HeLa cell with sensitive photoelectrochemical (PEC) feedback. Targeting the representative oncogenic miR‐21, the as‐functionalized nanopipette permits direct intracellular drug administration with precisely controllable dosages, and the corresponding therapeutic effects can be sensitively transduced by a PEC sensing interface that selectively responds to the indicator level of cytosolic caspase‐3. The experimental results reveal that injection of ca. 4.4 × 10 −20 mol miR‐21 inhibitor, i.e., 26488 copies, can cause the obvious therapeutic action in the targeted cell. This work features a solution to obtain the accurate knowledge of how a certain miR‐drug with specific dosages treats the cells and thus provides an insight into futuristic high‐precision clinical miR therapy using personalized medicine, provided that the prerequisite single‐cell experiments are courses of personalized customization.
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