作者
Zeshuai Wang,Zhaohui Wu,Hao Wang,Feng Ren,Guanlin Wang,M.M. Li,Shuang-Yin Wang,Xiaoyan Chen,Yiyi Su,Jun Wang,Weiwen Zhang,Yiliang Bao,Zhenwei Lan,Zhuo Song,Yiheng Wang,Xianyang Luo,Lu Zhao,Anfu Hou,Shuye Tian,Hejun Gao,Wenbin Miao,Yingyu Liu,Huilin Wang,Yin Chen,Zhi-Liang Ji,Mingqian Feng,Hongkun Liu,Lianghui Diao,Ido Amit,Yun Cao,Yonghui Zeng,Florent Ginhoux,WU Xi-sheng,Yue Zhu,Hanjie Li
摘要
Macrophages are heterogeneous and play critical roles in development and disease, but their diversity, function, and specification remain inadequately understood during human development. We generated a single-cell RNA sequencing map of the dynamics of human macrophage specification from PCW 4–26 across 19 tissues. We identified a microglia-like population and a proangiogenic population in 15 macrophage subtypes. Microglia-like cells, molecularly and morphologically similar to microglia in the CNS, are present in the fetal epidermis, testicle, and heart. They are the major immune population in the early epidermis, exhibit a polarized distribution along the dorsal-lateral-ventral axis, and interact with neural crest cells, modulating their differentiation along the melanocyte lineage. Through spatial and differentiation trajectory analysis, we also showed that proangiogenic macrophages are perivascular across fetal organs and likely yolk-sac-derived as microglia. Our study provides a comprehensive map of the heterogeneity and developmental dynamics of human macrophages and unravels their diverse functions during development.