A biomimetic 3D DNA nanoplatform for enhanced capture and high-purity isolation of stem cell exosomes

rolling circle amplification. Aptamers fold into specific secondary structures that bind to CD63, a protein expressed on the surface of exosomes, enabling efficient exosome capture. As a photothermal reagent, the temperature of the DNA nanoplatform increases under near-infrared light irradiation, destroying the secondary structure of the CD63 aptamer and releasing the exosomes. Additionally, we have demonstrated that a 3D DNA nanoplatform with multivalent CD63 aptamer structures achieves more efficient and convenient stem cell exosome separation compared to ultracentrifugation. This strategy provides an efficient and high-purity way to capture and reversibly separate exosomes, and the separated ultrapure exosomes are used for enhancing wound healing by modulating migration and angiogenesis.