CD14型
先天免疫系统
生物
病毒载量
炎症
趋化因子
CCL7型
免疫学
病毒学
免疫系统
病毒
CXCL10型
作者
Julien Clain,Henintsoa Rabezanahary,Gina Racine,Steven Boutrais,Calaiselvy Soundaramourty,Charles Joly Beauparlant,Mohammad‐Ali Jenabian,Arnaud Droit,Petronela Ancuța,Ouafa Zghidi-Abouzid,Jérôme Estaquier
出处
期刊:JCI insight
[American Society for Clinical Investigation]
日期:2023-07-24
卷期号:8 (14)
被引量:3
标识
DOI:10.1172/jci.insight.167856
摘要
Identifying immune cells and anatomical tissues that contribute to the establishment of viral reservoirs is of central importance in HIV-1 cure research. Herein, we used rhesus macaques (RMs) infected with SIVmac251 to analyze viral seeding in the liver and lungs of either untreated or early antiretroviral therapy-treated (ART-treated) RMs. Consistent with viral replication and sensing, transcriptomic analyses showed higher levels of inflammation, pyroptosis, and chemokine genes as well as of interferon-stimulating gene (ISG) transcripts, in the absence of ART. Our results highlighted the infiltration of monocyte-derived macrophages (HLA-DR+CD11b+CD14+CD16+) in inflamed liver and lung tissues associated with the expression of CD183 and CX3CR1 but also with markers of tissue-resident macrophages (CD206+ and LYVE+). Sorting of myeloid cell subsets demonstrated that CD14+CD206-, CD14+CD206+, and CD14-CD206+ cell populations were infected, in the liver and lungs, in SIVmac251-infected RMs. Of importance, early ART drastically reduced viral seeding consistent with the absence of ISG detection but also of genes related to inflammation and tissue damage. Viral DNA was only detected in CD206+HLA-DR+CD11b+ cells in ART-treated RMs. The observation of pulmonary and hepatic viral rebound after ART interruption reinforces the importance of early ART implementation to limit viral seeding and inflammatory reactions.
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