Anti-oxidative Effects of Taraxaci Herba and Protective Effects on Human HaCaT Keratinocyte

ABSTRACT Objectives ：This study was carried out to investigate anti-oxidative effect s of Taraxaci Herba (TH) and protective effects on Human HaCaT keratinocyte.Methods : Anti-oxidative effects were measured by estimating the amount o f total phenolics and flavonoids. In addition, DPPH free radical scavenging activitie s were estimated. Protective effects of TH on HaCaT keratinocytes against oxidative stress induced by h ydrogen peroxide were also measured. Results : In our results, treatment with TH did not show cytotoxicity on HaCaT keratinocyte beneath the concentration of 200 ㎍/㎖. 42.64±1.90 ㎍/㎖ of total phenolics and 28.09±1.84 ㎍/㎖ of flavonoids was detected from TH ethanol extract. In addition, D PPH free radical scavenging activities of TH were elevated in dose-dependent manner. In add ition, The value of half maximal inhibitory concentration (IC 50 ) was 165.5 ㎍/㎖. Finally, TH showed protective effect against cell death of HaCaT cell induced by hydrogen peroxide significantly.Conclusions : In conclusion, these results suggest that TH may have anti-oxidantic action in human skin and also suggest the possibility as cosmetic material.Key words : Taraxaci Herba; anti-oxidative effect; HaCaT keratinocyte