牙髓干细胞
成骨细胞
细胞生物学
骨钙素
骨桥蛋白
运行x2
骨结合蛋白
化学
干细胞
生物
碱性磷酸酶
免疫学
体外
生物化学
酶
作者
Giorgio Mori,Matteo Centonze,Giacomina Brunetti,Andrea Ballini,Angela Oranger,Cláudia Madalena Cabrera Mori,Luca Muzio,Stefano Tetè,F Ciccolella,Silvia Colucci,Maria Grano,Felice Roberto Grassi
出处
期刊:PubMed
日期:2010-05-22
卷期号:24 (2): 167-75
被引量:26
摘要
Stem cells are a promising tool for bone tissue regeneration. Dental pulp stem cells (DPSCs) can be easily obtained even in human young adults. In this study we investigated the capability of DPSCs, to express the osteoblastic phenotype when cultured with osteogenic medium. DPSCs isolated from the dental pulp of impacted third molar teeth were cultured with appropriate medium to induce osteoblast differentiation. Using Western-Blot, RT-PCR and microarray analysis, we studied the expression of osteoblastic parameter, and by Von Kossa staining we evaluated the production of mineralized matrix nodules. The results were compared with controls represented by undifferentiated DPSCs. DPSCs, differentiated into osteoblast-like cells, express large amount of alkaline phosphatase (ALP), collagen I (Coll I), osteopontin (OPN) and osteocalcin (OCN), all these parameters characterizing the osteoblastic phenotype. Differentiated DPSCs express Runx2 and JunB, a member of the AP-1 complex; both the transcription factors are associated with osteoblast differentiation and skeletal morphogenesis. Moreover, DPSCs express insulin growth factor-binding protein 5 (IGFBP-5), one of the regulating proteins of IGFs function. Finally, DPSCs can form mineralized matrix nodules that are a feature exclusive to osteoblasts. DPSCs could represent a potential source of osteoblasts to be used for bone regeneration.
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