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Restriction fragment length polymorphism study of Mycobacterium tuberculosis in Thailand using IS6110 as probe 相关领域
结核分枝杆菌
肺结核
医学
限制性片段长度多态性
北京
分子流行病学
基因分型
流行病学
基因型
病毒学
中国
遗传学
生物
基因
内科学
病理
地理
考古
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abstract: SETTING: Three referral hospitals in central Thailand. OBJECTIVE: To determine the population structure of Mycobacterium tuberculosis isolated from the referral hospitals. DESIGN: Study of 211 isolates of the bacteria received from the hospitals in central Thailand by Southern hybridization, with IS6110 probe and other probes when indicated. RESULTS: In 43 isolates only one copy of IS6110 was observed. These could be further differentiated by DR- and PGRS-specific probes. Two large groups of isolates with similar hybridization patterns were identified. The Beijing family, comprising 80 isolates, was previously reported to be commonly found in China, Mongolia, Thailand and Korea. CONCLUSION: Southern hybridization with IS6110 was found to be useful in studying the epidemiology of tuberculosis in Thailand. The existence of the Beijing family was confirmed. The unusually wide spread of the Beijing family in several countries in Asia merits further investigation. |
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(2025-6-4)