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Application of direct protein precipitation combined with tandem mass spectrometry in monitoring 5-fluorouracil therapeutic drugs in plasma
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By: Jin, Yongzeng; Sun, Liang; Li, Changkun; Ren, Biao; Hu, Yanyan To develop a method for the determination of 5-fluorouracil in plasma by direct protein precipitation coupled with tandem mass spectrometry. Plasma samples were treated with 0.1 mol·L-1 zinc sulfate as protein precipitator, and the supernatant was taken after centrifugation for direct determination Using 5-fluorouracil-13C, 15N2 as internal standards and Shimadzu shim pack GIST-HP C18-AQ (100 mm×2.1 mm I.D., 1.9μm) as chromatog. column; Water (phase A) - methanol (phase B) as mobile phase; Gradient elution: 0-1 min, 5%B; 1-2 min, 5%B→95%B; 2-2.5 min, 95%B; 2.5-2.6 min, 95%B→5%B; 2.6-5 min, 5%B; ****; Injection volume:*; Mass spectrometry quant. anal. was carried out by using ESI and ** (MRM); Detection ion pair: 5-fluorouracil m/z 129.1→42.1; 5-fluorouracil-13C, 15N2 internal standard m/z 132.1→44.1. Keywords: fluorouracil plasma tandem mass spectrometry direct protein precipitation; therapeutic drug monitoring |
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