| 标题 |
 Synthetic Intrinsically Disordered Proteins Enable Soluble Expression of Disulfide-Rich Therapeutics in E. coli |
| 网址 | |
| DOI | |
| 其它 | We previously reported the de novo design of three small (<20 kDa), highly soluble synthetic intrinsically disordered proteins (SynIDPs) and demonstrated their utility as solubility tags for proteins and antifouling agents. Building on this work, we now show that these hypersoluble SynIDPs significantly enhance the soluble expression of disulfide-rich proteins (DRPs) of therapeutic relevance, including fibroblast growth factor 21 (FGF-21), interleukin-15 (IL-15), and bovine pancreatic trypsin inhibitor (BPTI). Through SynIDP fusions, we achieve soluble recombinant production of functionally active DRPs containing a single disulfide bond in the E. coli strain BL21(DE3) and up to three nonconsecutive disulfide bonds in the E. coli SHuffle T7 Express strain, without the need for refolding. |
| 求助人 | |
| 下载 | 该求助完结已超 24 小时，文件已从服务器自动删除，无法下载。 |
PDF的下载单位、IP信息已删除
(2025-6-4)
水煮电吹风
Kao
唠叨的大门
GingerF
Criminology34
无极微光
OK
渡人舟
学术文献互助
YifanWang
v0id
null
Nole
秋风
潇洒的惋清
zjh33
罗Eason
乌鱼
skearthy
Anonymous
皖公网安备34019202002308
