| 标题 |
专利、报告等 CRISPR Cas9 system system for Bacillus subtilis genome edition and establishment method thereof
一种用于枯草芽孢杆菌基因组编辑的CRISPR Cas9系统及其建立方法
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Jun 15, 2016 发明人：吴敬，段绪果，张康 摘要：The invention discloses a CRISPR Cas9 system for Bacillus subtilis genome edition and an establishment method thereof, belonging to the technical field of gene engineering. A knock-out plasmid PHY300dsrf is established; and the plasmid comprises sgRNA and cas9 genes of the specific targeted target gene, a homologous restoration arm, and replication initial points and tolerance screening markers of Escherichia coli and Bacillus subtilis. The sgRNA of the specific targeted srfA-C gene can guide the cas9 protein to cut double strands at the specific site of the srfA-C gene, and can be used for accurate homologous recombination on the genome under the guide of the homologous restoration arm, thereby introducing the XhoI enzyme digestion site at the rupture. |
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