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Development and comparison of four real-time polymerase chain reaction systems for specific detection and quantification of zea mays l
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Abstract Four real-time polymerase chain reaction systems aiming at the specific detection and quantification of maize DNA are described. They have been developed in four independent laboratories targeting different maize sequences, i.e., alcohol dehydrogenase (Adh1), high mobility group protein (hmga), invertase A (ivr1), and zein, respectively. They were all fully specific, showing a very similar quantification accuracy along a number of distantly related maize cultivars and being either single or low copy number genes. They were highly sensitive and exhibited limits of quantification below 100 maize genomic copies. In consequence, they are considered suitable for use as maize specific endogenous reference genes in DNA analyses, including GMO quantitative tests. |
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(2025-6-4)
cdercder
Criminology34
FIN
OK
GingerF
无极微光
XX
Kao
秋风
归零者
null
斯文的白玉
kekekekekeke
Nole
My_magnum_opus
然宝
Chenmengdan
wonder
罗Eason
Contrail
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