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Facilitating secretory expression of apple seed -glucosidase Komagataella phaffii for the efficient preparation of salidroside |
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作者: Xin Yi Lu; Ming Yuan Lai; Peng Qin; Yu Cong Zheng; Jia Yi Liao; Zhi Jun Zhang; Jian He Xu; Hui Lei Yu Biotechnol J. 2024 Aug 摘要:Abstract Plant-derived B-glucosidases hold promise forglycoside biosynthesis via reverse hydrolysisbecause of their excellent glucose tolerance androbust stability. However, their poor heterologous expression hinders the development of large-scale production andapplications. In this study, we overexpressed 关键词: Komagataella phaffii;directed evolution;salidroside;secretory expression;β‐glucosidase |
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(2025-6-4)