透明质酸酶
材料科学
光动力疗法
体内
生物物理学
内吞作用
透明质酸
光热治疗
生物医学工程
癌症研究
作者
Wenjun Li,Cuifang Zheng,Zhengyin Pan,Chi Chen,Dehong Hu,Guanhui Gao,Shendong Kang,Haodong Cui,Ping Gong,Lintao Cai
出处
期刊:Biomaterials
[Elsevier]
日期:2016-09-01
卷期号:101: 10-19
被引量:83
标识
DOI:10.1016/j.biomaterials.2016.05.019
摘要
We here report smart hyaluronidase-actived theranostic nanoparticles based on hyaluronic acid (HA) coupled with chlorin e6 (Ce6) via adipic dihydrazide (ADH) forming HA-ADH-Ce6 conjugates and self-assembling into HACE NPs. The resulting nanoparticles showed stable nano-structure in aqueous condition with uniform size distribution and can be actively disassembled in the presence of hyaluronidase (over-expressed in tumor cells), exhibiting hyaluronidase-responsive "OFF/ON" behavior of fluorescence signal. The HACE NPs were rapidly taken up to human lung cancer cells A549 via CD44 (the HA receptor on the surface of tumor cells) receptor mediated endocytosis. Upon laser irradiation, the HACE NPs realized good near-infrared fluorescence imaging and photoacoustic imaging in the tumor bearing mice, which showed 5-fold higher fluorescence intensity and 3-fold higher photoacoustic (PA) intensity than free Ce6, respectively. In addition, under low dose of laser power, the HACE NPs presented more effective photodynamic therapy to suppression of tumor growth than free Ce6 in vitro and in vivo. Overall, these results suggest that the well-defined HACE NPs is a biocompatible theranostic nanoplatform for in vivo dual-modal tumor imaging and phototherapy simultaneously.
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