生物
间充质干细胞
细胞生物学
干细胞
重编程
细胞外基质
谱系(遗传)
祖细胞
弹性(物理)
基质(化学分析)
细胞命运测定
细胞
遗传学
材料科学
复合材料
作者
Adam J. Engler,Shamik Sen,H. Lee Sweeney,Dennis E. Discher
出处
期刊:Cell
[Elsevier]
日期:2006-08-01
卷期号:126 (4): 677-689
被引量:11568
标识
DOI:10.1016/j.cell.2006.06.044
摘要
Microenvironments appear important in stem cell lineage specification but can be difficult to adequately characterize or control with soft tissues. Naive mesenchymal stem cells (MSCs) are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity. Soft matrices that mimic brain are neurogenic, stiffer matrices that mimic muscle are myogenic, and comparatively rigid matrices that mimic collagenous bone prove osteogenic. During the initial week in culture, reprogramming of these lineages is possible with addition of soluble induction factors, but after several weeks in culture, the cells commit to the lineage specified by matrix elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types. Inhibition of nonmuscle myosin II blocks all elasticity-directed lineage specification-without strongly perturbing many other aspects of cell function and shape. The results have significant implications for understanding physical effects of the in vivo microenvironment and also for therapeutic uses of stem cells.
科研通智能强力驱动
Strongly Powered by AbleSci AI