Spermidine and 1,3-Diaminopropane Have Opposite Effects on the Final Stage of Cephalosporin C Biosynthesis in High-Yielding Acremonium chrysogenum Strain文献中结果中2.4最后俩段
Taking into account the comparative data on the expression of biosynthetic genes, the data after comparing the expression of the transport genes cefP, cefM, and cefT on two media turned out to be unexpected (Figure 8g–i). Both for the control SN medium and with the addition of PAs, after 24–48 h and until the end of fermentation, upregulation of all transport genes was observed, by 1.2–2.3 times, compared with the corresponding variants on CP media (Figure 7g–i, and Supplementary Materials Figure S2). This effect was both at the level that occurs when 1,3-DAP is added to the SN medium and in the range that distinguishes gene expression in the SN medium from gene expression in the CP medium (Figure 8). The expression of the key gene cefG, which is responsible for the conversion of DAC to CPC, was not changed compared to the addition of 1,3-DAP to the SN medium. Additionally , the expression of the transport gene CefT, whose work leads to the export of DAC from the cell, did not change compared with the addition of 1,3-DAP to the SN medium.
As a result, we can conclude that the disruption at the last stage of CPC biosynthesis caused by the addition of spermidine to the SN medium is not associated with disorder in the expression of biosynthetic and transport genes of beta-lactam BGCs.
翻译过来就是
(考虑到生物合成基因表达的比较数据,比较转运基因cefP、cefM和cefT在两种培养基上的表达后的数据是出乎意料的(图8g-i)。在对照SN培养基和添加PAs后,24-48 h至发酵结束,与CP培养基上的相应变异相比,所有转运基因均上调了1.2-2.3倍(图7g-i和补充资料图S2)。这种效应既与在SN培养基中添加1,3- dap时的水平相同,也处于SN培养基中基因表达与CP培养基中基因表达的区别范围内(图8)。与在SN培养基中添加1,3- dap相比,负责将DAC转化为CPC的关键基因cefG的表达没有变化。此外,与在SN培养基中添加1,3- dap相比,导致DAC从细胞输出的转运基因CefT的表达没有变化。
因此,我们可以得出结论,在SN培养基中添加亚精胺导致CPC生物合成最后阶段的中断与β -内酰胺BGCs生物合成和转运基因的表达紊乱无关。)
其中这加粗的文字,我就好奇是怎么得出没有变化的,而且前面补充材料S2给的也不是柱状图啊!!!!
图八
补充图S2,这图跟这个数据有关系吗?
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(2025-6-4)