氮气循环
计算生物学
生物
基因表达
基因
微生物代谢
基因组
遗传学
细菌
氮气
化学
有机化学
作者
James E. Graham,Nicholas B. Wantland,Mark Campbell,Martin G. Klotz
出处
期刊:Methods in Enzymology
日期:2011-01-01
卷期号:: 345-372
被引量:14
标识
DOI:10.1016/b978-0-12-386489-5.00014-2
摘要
Recent advances in DNA sequencing have greatly accelerated our ability to obtain the raw information needed to recognize both known and potential novel modular microbial genomic capacity for nitrogen metabolism. With PCR-based approaches to quantifying microbial mRNA expression now mainstream in most laboratories, researchers can now more efficiently propose and test hypotheses on the contributions of individual microbes to the biological accessibility of nitrogen upon which all other life depends. We review known microbial roles in these key nitrogen transformations, and describe the necessary steps in carrying out relevant gene expression studies. An example experimental design is then provided characterizing Nitrosococcus oceani mRNA expression in cultures responding to ammonia. The approach described, that of assessing microbial genome inventory and testing putative modular gene expression by mRNA quantification, is likely to remain an important tool in understanding individual microbial contributions within microbial community activities that maintain the Earth's nitrogen balance.
科研通智能强力驱动
Strongly Powered by AbleSci AI