Nanozymes for In Vitro Analysis

As a type of promising substitutes for natural enzymes, nanozymes have been widely used in in vitro analysis because of their unique advantages, including high stability, low cost, easy storage, multifunctionalities, among others. Bioanalysis based on the catalysis of nanozymes has attracted extensive research interest. This chapter begins with the principles of diverse detection techniques, including colorimetry, electrochemistry, chemiluminescence, electrochemiluminescence, fluorescence, and surface-enhanced Raman scattering. Representative advances in nanozyme-based bioanalysis in recent years are then introduced. Additionally, future challenges and perspectives are briefly discussed.