Carbon Stable Isotope Ratio of Dissolved Organic Matter as a Tool To Identify Its Sources and Transformations in a Tropical Montane Forest in Ecuador

Dissolved organic matter (DOM) contributes to forest C cycling. We assessed temporal variability, sources, and transformations of DOM during four years in a tropical montane forest with the help of stable C isotope ratios (δ¹³C values). We measured δ¹³C values of DOM in rainfall (RF), throughfall (TF), stemflow (SF), litter leachate (LL), soil solutions at the 0.15 and 0.30 m depths (SS15, SS30), and streamflow (ST) with TOC-IRMS. The δ¹³C values of DOM did not vary seasonally. We detected an event with a high δ¹³C value likely attributable to black carbon from local pasture fires. The mean δ¹³C values of DOM outside the event decreased in the order, RF (-26.0 ± 1.3‰) > TF (-28.7 ± 0.3‰) > SF (-29.2 ± 0.2‰) > LL (-29.6 ± 0.2‰) because of increasing leaching of C-isotopically light compounds. The higher δ¹³C values of DOM in SS15 (-27.8 ± 1.0‰), SS30 (-27.6 ± 1.1‰), and ST (-27.9 ± 1.1‰) than in the above-ground solutions suggested that roots and root exudates are major belowground DOM sources. Although in DOM the C/N ratios correlated with the δ¹³C values when all solutions were considered, this was not the case for SS15, SS30, and ST alone. Thus, the δ¹³C values of DOM provide an additional tool to assess the sources and turnover of DOM.