菠萝蛋白酶
阿纳纳斯
萃取(化学)
热重分析
傅里叶变换红外光谱
化学
醋酸
核化学
水解
响应面法
酶水解
色谱法
材料科学
酶
化学工程
生物化学
有机化学
植物
生物
工程类
蛋白酶
作者
Sajneen Akter Munmun,Taslim Ur Rashid,Md. Mizanur Rahman
标识
DOI:10.1016/j.jclepro.2024.140774
摘要
Plant-based enzymatic extraction of collagen from tannery rawhide trimmings can play an important role in the utilization tannery solid waste, providing economically viable and sustainable solutions. This study employed bromelain enzyme derived from pineapple (Ananas comosus) peel waste for collagen extraction and compared the procedure with conventional acetic acid extraction. The recovery of bromelain enzyme at 30–70% ethanol precipitation exhibited a 2.21-fold purification and a 98% yield. Response surface methodology (RSM) analysis revealed that bromelain enzyme-extracted collagen (BEC) yielded 82.20% at a hydrolysis time of 18.39 h, a mixing ratio of 22.38, and a temperature of 39.32 °C. In contrast, a lower yield of 66.94% was observed in the case of acetic acid-extracted collagen (AEC) at a hydrolysis time of 18.66 h, a mixing ratio of 19.41, and a temperature of 27.09 °C. Fourier transform infrared spectroscopy (FTIR) confirmed the successful extraction of both AEC and BEC. Thermogravimetric analysis (TGA) indicated the higher thermal stability of BEC compared to AEC. X-ray Diffraction (XRD) displayed characteristic peaks of collagen in the range of 2θ of 15–25°, affirming that BEC (sharp peak at 19.49°) is more crystalline than AEC (broadening peak at 19.88°). Elemental analysis revealed a higher nitrogen content (6.78%) in BEC, indicating a higher protein content than that of AEC (6.31%). Furthermore, antimicrobial activity, cytotoxicity evaluation, and heavy metal analysis revealed the potential applications of collagen in cosmetics, biomaterials, and tissue engineering.
科研通智能强力驱动
Strongly Powered by AbleSci AI